COOMASSIE BRILLIANT BLUE G 250 (C.I. 426

Code: 1154440025 D2-231

Analysis Note

Identity (UV/VIS-Spectrum): passes testAbsorption maximum λmax. (buffer pH 7.0): 577 - 584 nmSpec. Absorptivity A 1%/1cm (λmax; ...


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Analysis Note

Identity (UV/VIS-Spectrum): passes testAbsorption maximum λmax. (buffer pH 7.0): 577 - 584 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570TLC-Test: passes testLoss on drying (110 °C): ≤ 8 %Suitability for electrophoresis: passes test

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

Packaging

25 g in Glass bottle

bulk density520 kg/m3
InChI keyCMGWKYXJIYYESN-UHFFFAOYSA-M
InChI1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
pH6.4 (20 °C, 10 g/L in H2O)
potency>5000 mg/kg LD50, oral (Rat)
Quality Level100
solubility40 g/L
storage temp.no temp limit
technique(s)protein staining: suitable
Cas Number6104-58-1
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